Journal: Toxicon: X

Article Title: Venom system variation and the division of labor in the colonial hydrozoan Hydractinia symbiolongicarpus

doi: 10.1016/j.toxcx.2022.100113

Figure Lengend Snippet: Establishment of a Ncol-1::mScar transgenic. A) Schematic of donor plasmid created and injected into Hydractinia embryos; full coding domain sequence of a codon-optimized mScarlet-I is flanked by a putative promoter and terminator sequence of Ncol-1 obtained from the genome of Hydractinia . B) Live confocal image of full view of gastrozooid; strong signal in the central body column and tentacle regions. C) Close-up of tentacles in fixed gastrozooid. D) Close-up of the edge of the body column in fixed gastrozooid. E) Close-up of stolonal mat in growing colony; bottom shows fluorescent signal coming from Ncol-1 + cells. F) Branching stolon from the central colony shows strong fluorescence; inset shows the same tissue using light microscopy. G) Transgenic planula 24 hpf showing widespread Ncol-1 + cells, consistent with nematoblasts and recently matured nematocysts. B-D) Imaged using Leica Laser Scanning Confocal Upright microscope; E-G) Imaged using Lumenera Infinity camera with Infinity Analyze v7 software. Blue = DAPI, red = mScarlet. Scale bar: 200 μm (B, E-G), 100 μm (C), and 20 μm (D). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: B-D) Imaged using Leica Laser Scanning Confocal Upright microscope; E-G) Imaged using Lumenera Infinity camera with Infinity Analyze v7 software.

Techniques: Transgenic Assay, Plasmid Preparation, Injection, Sequencing, Fluorescence, Light Microscopy, Microscopy, Software